Food Research Institute

Alkhudhayri, D. A. – Osman, M. A. – Alshammari, G. M. – Aljehani, A. A. – Alghamdi, F. A. – AlFaris, N. A.
Comparative investigation of antioxidant content and activity of different parts of Moringa peregrina extracted by different solvent systems
Journal of Food and Nutrition Research, 65, 2026, No. 1, s. 38-49

Fatima A. Alghamdi, Department of Sports Health, College of Sports Sciences & Physical Activity, Princess Nourah bint Abdulrahman University, P. O. Box 84428, Riyadh 11671, Saudi Arabia. E-mail: Faaalghamdi@pnu.edu.sa

Original article
Received 22 October 2025; 1st revised 16 December 2025; accepted 26 January 2026; published online 8 February 2026.

DOI: https://doi.org/10.64122/SJUX1801

Summary: A comparative study evaluated the total polyphenol content (TPC), total flavonoid content (TFC), and antioxidant activity of Moringa peregrina (MP) seeds, bark, and leaves extracted using different solvents. Antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Significant variations were observed among plant parts and solvent systems. Leaves consistently showed the highest TPC and TFC, followed by bark and seeds. Absolute ethanol extracts yielded high TPC and TFC in leaves and bark, while aqueous methanol produced high TPC in bark. The strongest DPPH scavenging activity was found in leaves (91.6 %), followed by bark (90.7 %) and seeds (19.3 %). Ethanol extracts also showed the highest ABTS and FRAP antioxidant activity values, particularly in leaves and bark. Antioxidant activity was strongly correlated with phenolic and flavonoid contents regardless of the solvent used. Overall, MP leaves and bark demonstrated superior antioxidant potential, suggesting their possible therapeutic value in disease prevention and management.

Keywords: Moringa peregrina; polyphenols; ethanol; antioxidant activity

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