Food Research Institute

Zhou, L. – Pengmao, D. – Ma, X. – Jiao, L. – Ju, J.
Effect of selenium-containing exopolysaccharide extracted from Armillaria luteo-virens on physico-chemical and antioxidant properties of yogurt
Journal of Food and Nutrition Research, 59, 2020, No. 4, s. 341-351

Lianyu Zhou, Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Wusi xilu 38, 810008 Xining, China. E-mail: zly7604@163.com

Received 27 March 2020; 1st revised 25 September 2020; accepted 30 October 2020; published online 2 December 2020.

Summary: To improve the properties of yogurt, selenium-containing exopolysaccharide was added to milk. Orthogonal design was employed to optimize parameters of yogurt production based on physico-chemical indicators. Influence of the Se-containing exopolysaccharide on physico-chemical and antioxidant properties of yogurt were also studied. The optimum conditions were Se-containing exopolysaccharide 0.80 g·l^-1, saccharose 80 g·l^-1, inoculum 30 ml·l^-1 (3.00 × 10⁷ CFU·l^-1) and culture time 8 h, which led to production of yogurt with desired sensory characteristics, water holding capacity (WHC), counts of lactic acid bacteria (LAB), selenium concentration, and titration acidity. At optimal conditions, the Se-containing exopolysaccharide significantly improved sensory properties, WHC, selenium and soluble protein concentrations (P < 0.05), and significantly reduced titration acidity (P < 0.05). While Se-containing exopolysaccharide at 0.20 g·l^-1 significantly increased LAB counts, at 0.60–1.00 g·l^-1 a significant reduction in counts was observed in comparison to the control (P < 0.05). When compared with the control group, significantly (P < 0.05) enhanced removal of free radicals H₂O₂, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) was observed at 0.80–1.00 g·l^-1, 0.20–1.00 g·l^-1 and 0.20–1.00 g·l^-1 of Se-containing exopolysaccharide, respectively. Furthermore, significant correlations between physico-chemical and antioxidant properties were determined at P = 0.05 or P = 0.01.

Keywords: selenium-containing exopolysaccharide; yogurt; Armillaria luteo-virens; physico-chemical properties; antioxidant activity

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