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49 2010

Journal of Food and Nutrition Research
Súhrny čísla 4 / 2010

TRNČÍKOVÁ, T. – PISKERNIK, S. – KACLÍKOVÁ, E. – SMOLE MOŽINA, S. – KUCHTA, T. – JERŠEK, B.
Characterization of Staphylococcus aureus strains isolated from food produced in Slovakia and Slovenia with regard to the presence of genes encoding for enterotoxins
Journal of Food and Nutrition Research, 49, 2010, č. 4, s. 215-220

Barbara Jeršek, Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. E-mail: barbka.jersek@bf.uni-lj.si

Súhrn: Staphylococcus aureus strains containing genes encoding for enterotoxins are a problem from a food safety point of view as it may lead to the staphylococcal food poisoning in humans. In this study, 300 food samples were analysed for the presence of Staph. aureus, 38 Staph. aureus strains were isolated and analysed for the presence of genes encoding for SEA, SEB, SEC and SED using real-time polymerase chain reaction (PCR). Fourteen strains (37%) contained at least one gene encoding for SEA–SED, when 1 isolate (3%) was found positive for SEA, none was positive for SEB, 10 isolates (26%) were positive for SEC and 4 isolates (11%) were positive for SED. These rates are not very high, but the strains containing the respective genes represent a threat for food safety. The real-time PCR detection systems employed in our study proved to be a proper tool to fulfill the requirement for rapid and reliable screening of Staph. aureus strains isolated from food for genes encoding for the most frequent enterotoxins.

Kľúčové slová: Staphylococcus aureus; enterotoxin; food poisoning; real-time PCR

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