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46 2007

Journal of Food and Nutrition Research
Súhrny čísla 2 / 2007

MINAROVIČOVÁ, J. - KACLÍKOVÁ, E. - KRASCSENICSOVÁ, K. - SIEKEL, P.
Detection of Cryptosporidium parvum by polymerase chain reaction
Journal of Food and Nutrition Research, 46, 2007, č. 2, s. 58-62

Jana Minarovičová, Department of Microbiology and Molecular Biology, VÚP Food Research Institute, Priemyselná 4, P. O. Box 25, SK-82475 Bratislava 26, Slovakia. E-mail: petrikova@vup.sk

Súhrn: Cryptosporidium parvum is a protozoan parasite responsible for the outbreaks of cryptosporidiosis in humans. Several outbreaks of cryptosporidiosis associated with water and raw food occurred. Polymerase chain reaction (PCR) for direct detection of Cryptosporidium parvum oocysts in water, milk and apple juice was developed. Real-time PCR was able to detect 102 oocysts/ml in water, milk and apple juice. The sensitivity of PCR methods was improved using nested PCR, in which second round was modified by real-time PCR. In this case, the detection limit of nested PCR was less that 101 oocysts/ml of water, milk and apple juice, which compares favourably with the detection limit of the conventional PCR method - 103 oocyst/ml after DNA isolation by chaotropic solid phase extraction used in both cases.

Kľúčové slová: Cryptosporidium parvum; water; milk; apple juice; polymerase chain reaction

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