Vedecký časopis

Journal of Food and Nutrition Research
Online First Articles

Bitskinashvili, K. – Gabriadze, I. – Kutateladze, T. – Vishnepolsky, B. – Mikeladze, D. – Datukishvili, N.
Effects of thermal-acid treatment on degradation and amplification of wheat and maize DNA

Nelly Datukishvili, Department of Natural Sciences and Engineering, Ilia State University, Kakutsa Cholokashvili Ave. 3/5, 0162 Tbilisi, Georgia; Laboratory of Genome Structure and Function, Ivane Beritashvili Center of Experimental Biomedicine, Gotua St. 14, 0160 Tbilisi, Georgia. Tel.: +995 599949713; fax: +995 322231026, e-mail: neli_datukishvili@iliauni.edu.ge

Received 29 November 2017; 1st revised 31 January 2018; accepted 26 February 2018

Súhrn: Food processing causes DNA degradation and may hinder polymerase chain reaction (PCR) based detection of food components. Effects of heating (100 °C) and low pH (2 and 4) on degradation and amplification of maize and wheat DNA were investigated. DNA degradation was assessed by agarose gel electrophoresis and PCR amplification. PCR amplicons of different length ranging from 67 bp to 550 bp specific to plant chloroplast, maize or wheat genomes were used to monitor extent of DNA degradation. Combined thermal-acid treatment intensified time-dependent DNA degradation observed at thermal treatment and more significantly affected amplification than heating. Increase of acidity from pH 4 to pH 2 had negative influence on the integrity and amplifiability of genomic DNAs. PCR amplicons were degraded in a time- and size-dependent manner. Small DNA fragments ranging from 67 bp to 140 bp were amplified in all samples while 226 bp, 259 bp and 550 bp amplicons were not detected in certain highly processed samples. Our findings demonstrate that processing parameters such as temperature, pH and exposure time as well as amplicon size have crucial importance for DNA integrity and accurate detection of food components.

Kľúčové slová: thermally processed foods; acid treatment; Triticum aestivum; Zea mays; plant genomic DNA; polymerase chain reaction