Food Research Institute

AMIDŽIĆ KLARIĆ, D. – KLARIĆ, I. – MORNAR, A.
Polyphenol content and antioxidant activity of commercial blackberry wines from Croatia: Application of multivariate analysis for geographic origin differentiation
Journal of Food and Nutrition Research, 50, 2011, No. 4, s. 199-209

Daniela Amidžić Klarić, Department of Clinical Pharmacy, University Hospital Dubrava, Avenija Gojka Šuška 6, 10000 Zagreb, Croatia. Tel.: +385 1 290 3306; fax: +385 1 290 2906, e-mail: daniela.amidzic@gmail.com

Summary: Since polyphenolic compounds have been proposed to have beneficial effects on human health as antioxidants, the objectives of this study were to evaluate the polyphenol content and antioxidant activity in 17 commercial blackberry wines (BW) from three different Croatian subregions, and to investigate the relationship between these compounds and geographic origin of BW. A statistically significant difference was found between the concentration of monomeric anthocyanins present in the samples from three subregions. Furthermore, the concentrations obtained for gallic acid were significantly higher than for chlorogenic, caffeic and p-coumaric acids. The half-maximal inhibitory concentrations (IC50) were slightly higher for ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) test than for DPPH (2,2-diphenyl-1-picrylhydrazyl) test. Based on the results obtained by multivariate data analysis (principal component analysis and linear discriminant analysis) three models were created that gave a satisfactory grouping of the investigated BW based on their geographic origin. The best discriminating model showed not only extreme separation concerning subregions, but also a very narrow area of dispersion of individual wine samples intraregionally.

Keywords: blackberry wine; Rubus fruticosus; polyphenols; antioxidant activity; HPLC; multivariate

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